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This model fits the two IC50 values of the unlabelled ligand. It does not report the two Ki values. The Ki values depend on the IC50s, the concentration of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

IC50 is the concentration of agonist that gives a response half way between ... Depending on which units Y is expressed in, and the values of Bottom and Top, the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Relative vs absolute IC50; Fitting the absolute IC50; Incomplete dose-respone curves; Troubleshooting fits of dose-response curves. Dose-response - Stimulation ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The IC50. In many experiments, you vary the concentration of an inhibitor. With more inhibitor, the response decreases, so the dose-response curve goes ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

IC50 (inhibitory concentration) is the concentration of a drug that elicits ... In ideal cases, the dose-response curve extends between the control values, and ...

GraphPad FAQs

This symmetrical interval does a really poor job of quantifying the precision of the IC50. The simple fact is that the uncertainty is asymmetrical, and a ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You have a choice, in the Confidence tab, to either mark selected parameters as "unstable", to mark entire fits as "ambiguous", or neither.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

... IC50 IC60 IC70 IC75 IC80 IC90 ED10 ED20 ED25 ED30 ED40 ED50 ED60 ED70 ED75 ED80 ED90 LD10 LD20 LD25 LD30 LD40 LD50 LD60 LD70 LD75 LD80 LD90. Explore the ...

GraphPad FAQs

Are you fitting the relative IC50 but expecting an absolute IC50, and so are surprised by the results. © 1995-2025 GraphPad Software, LLC. All rights ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/