Manuals and step-by-step examples for Prism 5
Note that Prism 5 is several years old. If you use Prism 5, make sure you are up to date.
Manuals
Download the Prism 5 Statistics Help Guide (2.3MB pdf)
Download the Prism 5 Regression Guide (2.9MB pdf)
Access the Prism 5 Manual online
Getting Started
Step-by-Step Examples - Nonlinear Regression
Fitting an enzyme kinetics curve
Comparing two enzyme kinetics models
Interpolating from a sigmoidal standard curve
Automatic outlier elimination (exponential decay)
Global nonlinear regression (two dose-response curves)
Dependency and ambiguous fits (dose-response curves)
Detailed help to fit built-in equations
Prism comes with dozens of built-in equations to use in nonlinear regression (of course you can also add your own equations). For each of the built-in equations, we provide a detailed description with step-by-step instructions. Here are a few examples:
Equation: log(agonist) vs. response -- Variable slope
Equation: log(inhibitor) vs. normalized response
Equation: One phase exponential decay
Equation: Competitive binding - One site - Fit Ki
Equation: Receptor binding - Dissociation kinetics
Equation: Mixed-model enzyme inhibition
Equation: Gaddum/Schild EC50 shift
Step-by-Step Statistical Examples
Descriptive Statistics
Compare two groups
Unpaired t test from averaged data
Compare three or more groups
Repeated measures one-way ANOVA
Two-way ANOVA
Two-way ANOVA. Repeated measures by row
Two-way ANOVA. Repeated measures by column
Categorical outcomes
Diagnostic lab tests