KNOWLEDGEBASE - ARTICLE #1825
Plateau followed by two phase association
The Plateau followed by one phase association equation is included in the Prism equation list. This equation can be adapted to describe a two phase association and will produce the following type of curve:
This is the equation:
SpanFast=(Plateau-Y0)*(PercentFast*.01)
SpanSlow=(Plateau-Y0)*(100-PercentFast)*.01
Y2 = Y0+ (SpanFast*(1-exp(-KFast*(X-X0)))) + (SpanSlow*(1-exp(-KSlow*(X-X0))))
Y= IF( X<X0, Y0, Y2)
In this equation, the definition of the parameters are:
Plateau and Y0 are both the same units as Y
X and X0 both represent Time
PercentFast is the percent of the signal that is due to the fast phase
KFast and KSlow are rate constants that are reciprocal of X axis units
Click here to download the example file that includes this equation.