How do I handle data with mores replicates than Prism can accept.

Prism 6 and later allow 256 side-by-side subcolumns, while Prism 4 and Prism 5 only allowed  52 side-by-side replicates.

• For one-way ANOVA, column scatter or box-and-whisker plots, or column bar graphs, start with a column data table and enter your data with the replicates stacked in a column, rather than in subcolumns. This is not a solution when you have X values and you want to fit curves or draw XY graphs, or if you are performing two-way ANOVA.

 

• For an XY or grouped graph, enter the mean, SD (or SEM) and N, calculated elsewhere.

•  In Prism 3, to get 17-52 replicates, format the table for more than 16 replicates.

 

 

 

 

 

 

 

Now you have only one data group per table. That's not a limitation for graphing--you can superimpose any number of tables on one graph--but it is a big limitation for many analyses.