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After fitting the log of the EC50, Prism does the antilogarithm transform to report the EC50 and its logarithm. Prism assumes that you used base 10 ...

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The EC50, fit by standard dose-response models, is determined by two properties of the agonist: •How well it binds to the receptor, quantified by the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You may get a narrow confidence interval that way. For example, this page explains how to fit the ratio of two EC50 values of dose-response curves.

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In this case, use nonlinear regression to quantify the fold shift in the EC50 and its confidence interval, and to compute a P value that tests the null ...

https://www.graphpad.com/guides/prism/latest/statistics/

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

In this case, the equation fits the EC50, rather than the LogEC50. However, for both sets of equations, Prism will report both the LogEC50 and the transformed ...

GraphPad FAQs

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The parameter Ka is the EC50, the concentration that provokes a response halfway between Bottom and Top. As written, that equation includes the parameter ...

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Khalf is the concentration of substrate that produces a half-maximal enzyme velocity. It is the EC50. h is the Hill slope. When h=1, this equation is identical ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: EC50 shift; Equation: Allosteric EC50 shift; Equation: ECanything; Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]) ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/