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Enter the data with the technical replicates stacked. In the example, you tested between 4 and 6 students in each room and these are stacked in each subcolumn.

https://www.graphpad.com/guides/prism/latest/statistics/

... stacked, interleave, grouped, or superimposed. ... If your data table has only one row, Prism will place the column titles, rather than row titles, under each bar ...

https://www.graphpad.com/guides/prism/latest/user-guide/

Click Analyze, look in the list of Column analyses, and choose Analyze a stack of P values. Choose one of two general approaches: Control the False Discovery ...

https://www.graphpad.com/guides/prism/latest/statistics/

Place computed unknown values into the X column, with replicate values stacked ... In this case, Prism creates two tabs of results. The interpolation is the same ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

But when you identify outliers in a stack of column data, Prism does not automatically identify those outliers on the graph. ... On the column table, each group ...

GraphPad FAQs

Column tables offer no choices for data entry A Column table has no subcolumns. Replicate values are stacked into each column. Note the distinction between ...

https://www.graphpad.com/guides/prism/7/user-guide/

AND Each row represents a different time point, so matched values are stacked into a subcolumn. Disabled multiple comparisons test: Compare column means (main ...

GraphPad FAQs

Enter replicate values stacked into columns. Enter the data for each group into a separate column. The two groups do not have be the same size (it's OK to ...

https://www.graphpad.com/guides/prism/latest/statistics/

Prism 5 and earlier versions doesn't let you make a graph with bars or columns and also show the individual data points as a scatter plot. The workaround is to ...

GraphPad FAQs

So all values from each group are stacked into a column. Prism will perform a one-sample t test (or Wilcoxon rank sum test) on each column you enter. 3 ...

https://www.graphpad.com/guides/prism/latest/statistics/