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The EC50 is the concentration of a drug that gives half-maximal response. The IC50 is the concentration of an inhibitor where the response (or binding) is ...

GraphPad FAQs

AbsoluteIC50 is the concentration of antagonist that gives a response half way between Top and Baseline.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Any attempt to determine an IC50 by fitting a curve to the data in the graph above will be useless. Prism might, or might not, be able to fit a ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The relative and absolute IC50 values are the same when the curve descends to a Y value of 0. In many cases, you have reliable control data to define 0. In ...

GraphPad FAQs

The IC50 is the concentration of an inhibitor where the response (or binding) is reduced by half. Seems simple enough. But when you actually go to fit data to ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

This EPA document gives the needed equation (which I have generalized a bit, so does not require that the data already be normalized).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

From the data table, click Analyze, choose nonlinear regression, and choose the panel of equations: Dose-Response -- Special, X is log(concentration). Then ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

IC50 and EC50 are, respectively, the inhibition and effective concentrations that produce 50% of maximal response from a drug or treatment dose.

GraphPad FAQs

response curves follow the familiar symmetrical sigmoidal shape. The goal is to determine the IC50 of the inhibitor - the concentration that provokes a response ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

IC50 is the concentration of agonist that gives a response half way between ... Depending on which units Y is expressed in, and the values of Bottom and Top, the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/