•The t test (and nonparametric) analysis compares two data set columns. Each set of replicate values are usually entered into a column, although Prism can also enter replicates entered into side-by-side subcolumns all on one row.
•The multiple t test analysis performs many t tests at once -- one per row. Replicates are entered into side-by-side subcolumns.
1.Create a Grouped data table. Format the table either for entry of replicate values into subcolumns, or for entry of mean, SD (or SEM) and n.
2.Enter the data on two data set columns. One unpaired t test will be performed on each row of data.
3.Click Analyze, and choose "Multiple t tests -- one per row" from the list of analyses for Grouped data.