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This guide is for an old version of Prism. Browse the latest version or update Prism

The results are presented on two pages.

t tests page

This page shows the t test for each row. The P value for each comparison is shown, and also the multiplicity adjusted P value, which is higher.  

If you chose the statistical significance approach, the first column is  labeled "Significant?" and provides a "Yes" or "No" to indicate if the comparison was declared to be statistically significant, after any adjustments you requested for multiple comparisons. In prior versions of Prism, this column either contained a single asterisk (to indicate that a comparison was declared statistically significant) or not. However, it never showed several asterisks regardless of the corresponding P value. To avoid confusion, this notation was replaced with a simple "Yes" or "No".

If you chose the FDR approach, then the first column is labeled "Discovery?" and contains an asterisk for those rows deemed to be "discoveries" and otherwise is blank.

Significant results page or Discoveries page

This page only shows the rows whose results meet the definition of a "statistically significant result" or a "discovery" based on your choices in the dialog. The rows are sorted by P value, with the smallest P value on top.

What is n?

In most cases, you have complete data on each row and the number of t tests equals the number of rows, so there is no ambiguity.

In some cases, it may not be possible to compute the t test for some rows (because the SD of the replicates is 0.0, or because there is only one value for each group). Prism only counts the rows for which a t test could be computed. If a t test could not be computed for some rows, Prism points this out in a floating note.

 

 

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