In prior versions of Prism, the one-sample t test and the Wilcoxon rank sum tests were computed as part of Prism's Column Statistics analysis. Starting with Prism 8, there is a separate analysis in Prism for these tests.  

1.Create a Column data table.

2.Enter each data set in a single Y column. So all values from each group are stacked into a column. Prism will perform a one-sample t test (or Wilcoxon rank sum test) on each column you enter.

3.Click Analyze, look in the list of Column analyses, and choose one-sample t and Wilcoxon test.