The one-sample t test and the Wilcoxon rank sum tests are computed as part of Prism's Column Statistics analysis. Follow these steps

1.Create a Column data table.

2.Enter each data set in a single Y column. So all values from each group are stacked into a column. Prism will perform a one-sample t test (or Wilcoxon rank sum test) on each column you enter.

3.Click "Analyze... Statistical analyses... Column statistics"

4. At the bottom of the Column Statistics dialog, in the section labeled Inferences, check the option to perform either the one-sample t test or the Wilcoxon rank sum test.

5.To the right of that option, enter the hypothetical value to which you wish to compare the mean (t test) or median (Wilcoxon test). This value is often 0, or 100 (when values are percentages), or 1.0 (when values are ratios).