GraphPad Statistics Guide

How the Fisher LSD method works

How the Fisher LSD method works

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How the Fisher LSD method works

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The Fisher's LSD test works just like a t test with one exception. A t test computes a pooled standard deviation from the two groups being compared. The Fisher LSD test uses the pooled standard deviation. This pooled SD comes from all the groups, which gives it more degrees of freedom and more power.

Note that the Fisher's LSD test does not account for multiple comparisons. It is rarely recommended.