GraphPad Curve Fitting Guide

Enzyme kinetics -- Inhibition

Enzyme kinetics -- Inhibition

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Enzyme kinetics -- Inhibition

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Many drugs work by inhibiting enzyme activity, either by preventing the substrate from binding to the enzyme, or by stabilizing the enzyme-substrate complex so as to slow formation of product.To distinguish between the models of enzyme inhibition and determine the Ki of the inhibitor, measure substrate-velocity curves in the presence of several concentrations of inhibitor (including one curve with no inhibitor).