1. Enter the unknown values on the same table as your standard curve. Just below the standard curve values, enter your unknowns as Y without X. This example shows X and Y values for five standards, and Y values for four unknowns.
2. Click on the Graphs button, and look at a graph of your standard curve.
3. Click the analyze button, and choose how to fit the standard curve. Choose either linear or nonlinear regression, or create a LOWESS, Spline, or Point-to-point curve.
4. On the Parameters dialog, check the option to perform standard curve calculations.
5. Click on the Results button to see the tabular results. Drop down the View list and pick the standard curve.
6. Look at the table of X and Y values. The Y column contains values you entered, and the X column shows the calculated concentrations in the same units as you used for the X axis.
7. If necessary, transform the results to anitlogs. Click the Analyze button. On the New Analysis dialog, choose Transforms and choose to analyze the data you are looking at.
