In response to numerous requests, I've posted a short article explaining how to use Prism to fit enzyme kinetic data with competitive or uncompetitive inhibition. That article links to a Prism file that fits one family of data sets to a competitive, noncompetitive, and uncompetitive model of inhibition.

If you want to learn more about this field, the new boook by Copeland explains the chemistry, biology and math extremely well.